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array based oligonucleotide synthesis  (Twist Bioscience)


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    Structured Review

    Twist Bioscience array based oligonucleotide synthesis
    Array Based Oligonucleotide Synthesis, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/oligonucleotide+array/array+based+oligonucleotide+synthesis/pmc13174209-313-7-10
    Average 86 stars, based on 1 article reviews
    array based oligonucleotide synthesis - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Synthesized:

    Article Title: Synthetic introns enable splicing factor mutation-dependent targeting of cancer cells.
    Article Snippet: Many cancers carry recurrent, change-of-function mutations affecting RNA splicing factors.. Here, we describe a method to harness this abnormal splicing activity to drive splicing factor mutationdependent gene expression to selectively eliminate tumor cells.. We engineered synthetic introns that were efficiently spliced in cancer cells bearing SF3B1 mutations, but unspliced in otherwise isogenic wild-type cells, to yield mutation-dependent protein production.

    Amplification:

    Article Title: Small DNA elements that act as both insulators and silencers in plants
    Article Snippet: The synthetic insulators sIns1 and sIns2 were PCR amplified from pZS*11_4enh (Addgene no. 149423; https://www.addgene.org/149423/ ; ref. ) and pEvolvR-enCas9-PolI3M-TBD (Addgene no. 113077; https://www.addgene.org/113077/ ; ref. ), respectively. .. Insulator fragments were ordered as an oligonucleotide array from Twist Bioscience with 15-bp flanking sequences for amplification. ..

    Article Title: Plant enhancers exhibit both cooperative and additive interactions among their functional elements
    Article Snippet: .. Single-nucleotide and double-deletion variants of the 3 plant enhancers with 15-bp flanking sequences (5′ TAACTCGCCTCGATC and 3′ CCGTGACAGGTCATT for AB80 ; 5′ ACATGGGGGATCATG and 3′ GCATTAGCCAGTCTG for Cab-1 ; 5′ GCTCCAGTTCCCAAC and 3′ GATCGTCCAGTCTGA for rbcS-E9 ) for amplification were ordered as an oligonucleotide array from Twist Bioscience. .. Enhancer fragments ( ) with 5′ GTGATG overhangs and their reverse-complements with 5′ CATCAC overhangs were ordered as oligonucleotides, annealed, and 5′ phosphorylated with T4 Polynucleotide Kinase (NEB).

    Article Title: Small DNA elements can act as both insulators and silencers in plants
    Article Snippet: The synthetic insulators sIns1 and sIns2 were PCR amplified from pZS*11_4enh (Addgene no. 149423; https://www.addgene.org/149423/ ) ( ) and pEvolvR-enCas9-PolI3M-TBD (Addgene no. 113077; https://www.addgene.org/113077/ ) , respectively. .. Insulator fragments were ordered as an oligonucleotide array from Twist Bioscience with 15 bp flanking sequences for amplification. ..

    Article Title: Plant enhancers exhibit both cooperative and additive interactions among their functional elements.
    Article Snippet: .. Single-nucleotide and double-deletion variants of the 3 plant enhancers with 15-bp flanking sequences (5′ TAACTC GCCTCGATC and 3′ CCGTGACAGGTCATT for AB80; 5′ ACATGGGGGATCATG and 3′ GCATTAGCCAGTCTG for Cab-1; 5′ GCTCCAGTTCCCAAC and 3′ GATCGTCCAGT CTGA for rbcS-E9) for amplification were ordered as an oligonucleotide array from Twist Bioscience. .. Enhancer fragments (Fig. 6A) with 5′ GTGATG overhangs and their reverse-complements with 5′ CATCAC overhangs were ordered as oligonucleotides, annealed, and 5′ phosphorylated with T4 Polynucleotide Kinase (NEB).

    Biomarker Discovery:

    Article Title: Plant enhancers exhibit both cooperative and additive interactions among their functional elements.
    Article Snippet: .. Enhancer fragment combinations for the validation library 12 | THE PLANT CELL 2024: Page 1 of 17 Jores et al. D ow nloaded from https://academ ic.oup.com /plcell/advance-article/doi/10.1093/plcell/koae088/7633284 by Birm ingham C ity U niversity user on 10 April 2024 were ordered as an oligonucleotide array from Twist Bioscience. .. All libraries used in this study were constructed using pPSup (Addgene no. 149416; https://www.addgene.org/ 149416/; Jores et al. 2020) as the base plasmid.

    Article Title: Plant enhancers exhibit both cooperative and additive interactions among their functional elements
    Article Snippet: .. Enhancer fragment combinations for the validation library were ordered as an oligonucleotide array from Twist Bioscience. .. All libraries used in this study were constructed using pPSup (Addgene no. 149416; https://www.addgene.org/149416/ ; ) as the base plasmid.



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